The determination of the total amount of saturated & unsaturated fat is traditionally carried out by a procedure known as FAMES (Fatty acid methyl ester analysis). Fat is traditionally extracted from samples using a cold solvent extraction into solvents such as DCM or other petroleum based solvents. Extracts are then dried and derivatized to form fatty acid methyl esters using toxic reagents such as boron trifluoride. This process is both lengthy and hazardous. Microwave assisted FAME’s replicates the AOAC method but in a much safer, cleaner and more efficient way. It creates exactly the same methyl esters that ultimately quantified by Gas Chromatography.